LS-B10235

antibody from LSBio

Targeting: VHL VHL1

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B10235

Provider

LSBio

Product name

IHC-plus™ VHL / Von Hippel Lindau Antibody (aa167-179) LS-B10235

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Rat, Canine, Rabbit, Simian

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

VHL antibody (0.01 ug/ml) staining of Human Ovary lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

VHL / Von Hippel Lindau antibody (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

VHL / Von Hippel Lindau antibody (1.5ug/ml) as the reporter with EB002014 as the capture rabbit antibody (2.5ug/ml).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

VHL / Von Hippel Lindau antibody (1.5ug/ml) as the reporter with EB002014 as the capture rabbit antibody (2.5ug/ml).