H00007428-M01

antibody from Abnova Corporation

Targeting: VHL VHL1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007428-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007428-M01, RRID:AB_509115

Product name

VHL monoclonal antibody (M01), clone 1G12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant VHL.

Antigen sequence

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGA
EESGPEESGPEELGAEEEMEAGRPRPVLRSVNSRE
PSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGT
GRRIH


Isotype

IgG

Antibody clone number

1G12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of VHL expression in transfected 293T cell line by VHL monoclonal antibody (M01), clone 1G12.Lane 1: VHL transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged VHL is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to VHL on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol