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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB1074 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1074, RRID:AB_1581853
- Product name
- VHL monoclonal antibody, clone 52A11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant VHL.
- Isotype
- IgG
- Antibody clone number
- 52A11
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Chemokine receptor CXCR4 downregulated by von Hippel-Lindau tumour suppressor pVHL.
The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis.
Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function.
Staller P, Sulitkova J, Lisztwan J, Moch H, Oakeley EJ, Krek W
Nature 2003 Sep 18;425(6955):307-11
Nature 2003 Sep 18;425(6955):307-11
The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis.
Maxwell PH, Wiesener MS, Chang GW, Clifford SC, Vaux EC, Cockman ME, Wykoff CC, Pugh CW, Maher ER, Ratcliffe PJ
Nature 1999 May 20;399(6733):271-5
Nature 1999 May 20;399(6733):271-5
Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function.
Stebbins CE, Kaelin WG Jr, Pavletich NP
Science (New York, N.Y.) 1999 Apr 16;284(5413):455-61
Science (New York, N.Y.) 1999 Apr 16;284(5413):455-61
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of HepG2 cell lysate was resolved by SDS - PAGE , transferred to PVDF membrane and probed with VHL monoclonal antibody , clone 52A11 (1 : 500) (Cat # MAB1074) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
