14-7888-82

IRF8 antibody from Invitrogen Antibodies
ICSBP, ICSBP1, IRF-8
Western blot
Supportive data in Antibodypedia
Flow cytometry
Recommended by provider

Antibody data

Product number
14-7888-82
Provider
Invitrogen Antibodies
Product name
Anti-IRF8 Monoclonal Antibody (GW4CML3), eBioscience™
Provider product page
Invitrogen Antibodies - 14-7888-82
Antibody type
Monoclonal
Antigen
Other
Description
Description: The GW4CML3 monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC. IRF8-/- mice are deficient in both pDC and CD8a+ DC populations. CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation. IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice. Applications Reported: This GW4CML3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated GW4CML3 is recommended for use in intracellular flow cytometry). Applications Tested: This GW4CML3 antibody has been tested by western blot on lysates from stimulated normal human peripheral blood cells. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Mouse
Isotype
IgG
Antibody clone number
GW4CML3
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
Provider Type Product Number
- No reagents -