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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- AF3508 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Lipocalin-2/NGAL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.
- Reactivity
- Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis.
Expression and localisation of brain-type organic cation transporter (BOCT/24p3R/LCN2R) in the normal rat hippocampus and after kainate-induced excitotoxicity.
Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
Asimakopoulou A, Borkham-Kamphorst E, de Leur EV, Weiskirchen R
Data in brief 2017 Aug;13:644-649
Data in brief 2017 Aug;13:644-649
Expression and localisation of brain-type organic cation transporter (BOCT/24p3R/LCN2R) in the normal rat hippocampus and after kainate-induced excitotoxicity.
Chia WJ, Tan FC, Ong WY, Dawe GS
Neurochemistry international 2015 Aug;87:43-59
Neurochemistry international 2015 Aug;87:43-59
Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
Soler-García AA, Johnson D, Hathout Y, Ray PE
Clinical journal of the American Society of Nephrology : CJASN 2009 Apr;4(4):763-71
Clinical journal of the American Society of Nephrology : CJASN 2009 Apr;4(4):763-71
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse and Rat Lipocalin-2/NGAL by Western Blot. Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipocalin-2/NGAL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
