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- Antibody Data
- Antigen structure
- References [7]
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- Western blot [1]
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- Product number
- MAB1857-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Lipocalin-2/NGAL Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant mouse (rm)Lipocalin-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhLipocalin-1 or rhLipocalin-2.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P11672- Isotype
- IgG
- Antibody clone number
- 228418
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Fibrin Cleavage Product Bβ15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury.
Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment.
Neutrophil gelatinase‑associated lipocalin as a potential novel biomarker for ventilator‑associated lung injury.
Differential role of lipocalin 2 during immune complex-mediated acute and chronic inflammation in mice.
Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics.
Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2.
Interferon-gamma limits the availability of iron for intramacrophage Salmonella typhimurium.
Fischer D, Seifen C, Baer P, Jung M, Mertens C, Scheller B, Zacharowski K, Hofmann R, Maier TJ, Urbschat A
Frontiers in pharmacology 2018;9:369
Frontiers in pharmacology 2018;9:369
Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment.
Gomez-Chou SB, Swidnicka-Siergiejko AK, Badi N, Chavez-Tomar M, Lesinski GB, Bekaii-Saab T, Farren MR, Mace TA, Schmidt C, Liu Y, Deng D, Hwang RF, Zhou L, Moore T, Chatterjee D, Wang H, Leng X, Arlinghaus RB, Logsdon CD, Cruz-Monserrate Z
Cancer research 2017 May 15;77(10):2647-2660
Cancer research 2017 May 15;77(10):2647-2660
Neutrophil gelatinase‑associated lipocalin as a potential novel biomarker for ventilator‑associated lung injury.
Xiao R, Chen R
Molecular medicine reports 2017 Jun;15(6):3535-3540
Molecular medicine reports 2017 Jun;15(6):3535-3540
Differential role of lipocalin 2 during immune complex-mediated acute and chronic inflammation in mice.
Shashidharamurthy R, Machiah D, Aitken JD, Putty K, Srinivasan G, Chassaing B, Parkos CA, Selvaraj P, Vijay-Kumar M
Arthritis and rheumatism 2013 Apr;65(4):1064-73
Arthritis and rheumatism 2013 Apr;65(4):1064-73
Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics.
te Boekhorst BC, Bovens SM, Hellings WE, van der Kraak PH, van de Kolk KW, Vink A, Moll FL, van Oosterhout MF, de Vries JP, Doevendans PA, Goumans MJ, de Kleijn DP, van Echteld CJ, Pasterkamp G, Sluijter JP
Cardiovascular research 2011 Feb 15;89(3):680-8
Cardiovascular research 2011 Feb 15;89(3):680-8
Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2.
Nairz M, Theurl I, Schroll A, Theurl M, Fritsche G, Lindner E, Seifert M, Crouch ML, Hantke K, Akira S, Fang FC, Weiss G
Blood 2009 Oct 22;114(17):3642-51
Blood 2009 Oct 22;114(17):3642-51
Interferon-gamma limits the availability of iron for intramacrophage Salmonella typhimurium.
Nairz M, Fritsche G, Brunner P, Talasz H, Hantke K, Weiss G
European journal of immunology 2008 Jul;38(7):1923-36
European journal of immunology 2008 Jul;38(7):1923-36
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Lipocalin-2/NGAL by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
