Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB1757-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Lipocalin-2/NGAL Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Lipocalin-1 or rmLipocalin-2. This antibody also recognizes human Lipocalin-2/MMP-9 complexes in Western blots under non-reducing conditions.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P80188
- Isotype
- IgG
- Antibody clone number
- 220310
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies.
Reactive astrocytes secrete lcn2 to promote neuron death.
Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Urinary biomarkers predict brain tumor presence and response to therapy.
Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis.
An S, Raju I, Surenkhuu B, Kwon JE, Gulati S, Karaman M, Pradeep A, Sinha S, Mun C, Jain S
The ocular surface 2019 Jul;17(3):589-614
The ocular surface 2019 Jul;17(3):589-614
Reactive astrocytes secrete lcn2 to promote neuron death.
Bi F, Huang C, Tong J, Qiu G, Huang B, Wu Q, Li F, Xu Z, Bowser R, Xia XG, Zhou H
Proceedings of the National Academy of Sciences of the United States of America 2013 Mar 5;110(10):4069-74
Proceedings of the National Academy of Sciences of the United States of America 2013 Mar 5;110(10):4069-74
Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
Mir SU, Jin L, Craven RJ
The Journal of biological chemistry 2012 Apr 27;287(18):14494-501
The Journal of biological chemistry 2012 Apr 27;287(18):14494-501
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
Urinary biomarkers predict brain tumor presence and response to therapy.
Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Apr 15;14(8):2378-86
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Apr 15;14(8):2378-86
Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis.
Pitashny M, Schwartz N, Qing X, Hojaili B, Aranow C, Mackay M, Putterman C
Arthritis and rheumatism 2007 Jun;56(6):1894-903
Arthritis and rheumatism 2007 Jun;56(6):1894-903
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Lipocalin-2/NGAL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Lipocalin-2/NGAL by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and human bone marrow tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lipocalin-2/NGAL in Human Pancreas. Lipocalin-2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.