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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PA5-88079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NGAL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QDSTSDLIPA PPLSKVPLQQ NFQDNQFQGK WYVVGLAGNA ILREDKDPQK MYATIYELKE DKSYNVTSVL FRKKKCDYWI RTFVPGCQPG EFTLGNIKSY PGLTSYLVRV VSTNYNQHAM VFFKKVSQNR EYFKITLYGR TKELTSELKE NFIRFSKSLG LPENHIVFPV PIDQCIDG; Positive Samples: A-549
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Lipocalin-2 and Calprotectin Potential Prognosis Biomarkers in Peripheral Arterial Disease.
Saenz-Pipaon G, Ravassa S, Larsen KL, Martinez-Aguilar E, Orbe J, Rodriguez JA, Fernandez-Alonso L, Gonzalez A, MartÃn-Ventura JL, Paramo JA, Lindholt JS, Roncal C
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2022 Apr;63(4):648-656
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2022 Apr;63(4):648-656
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NGAL in extracts of various cell lines. Samples were incubated with NGAL Polyclonal antibody (Product # PA5-88079) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NGAL was performed in A-549 cells using NGAL Polyclonal Antibody (Product # PA5-88079). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NGAL in A-549 cells. Samples were incubated with NGAL Polyclonal antibody (Product # PA5-88079). Blue: DAPI for nuclear staining.
