609707

antibody from BioLegend

Targeting: SSRP1 FACT80

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

609707

Provider

BioLegend

Proper citation

BioLegend Cat#609707, RRID:AB_2566463

Product name

Alexa Fluor® 647 anti-SSRP1

Antibody type

Monoclonal

Antigen

Recombinant (partial) (308-524aa)

Description

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D1 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Red dye

Antibody clone number

10D1

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were treated with True-Nuclear™ Transcription Factor Buffer Set and then stained with anti-SSRP-1 (clone 10D1) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).

Conjugate

Red dye