609808

antibody from BioLegend

Targeting: SSRP1 FACT80

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

609808

Provider

BioLegend

Proper citation

BioLegend Cat#609808, RRID:AB_2566301

Product name

Alexa Fluor® 647 anti-SSRP1

Antibody type

Monoclonal

Antigen

Recombinant (partial)

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Antibody clone number

3.00E+04

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the cells were intracellularly stained with 2.5 µg/ml anti-SSRP1 (clone 3E4) Alexa Fluor® 647 (red) in blocking buffer overnight at 4°C, followed by phalloidin staining (green) for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

Conjugate

Red dye