609812

antibody from BioLegend

Targeting: SSRP1 FACT80

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

609812

Provider

BioLegend

Proper citation

BioLegend Cat#609812, RRID:AB_2566636

Product name

Brilliant Violet 421™ anti-SSRP1

Antibody type

Monoclonal

Antigen

Recombinant (partial)

Reactivity

Human

Host

Mouse

Conjugate

Blue dye

Antibody clone number

3.00E+04

Vial size

100 µg

Concentration

0.2 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml anti-SSRP1 (clone 3E4) Brilliant Violet 421™ (blue) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DRAQ5 (red). The image was captured with a 40X objective.

Conjugate

Blue dye