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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 609802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#609802, RRID:AB_2195751
- Product name
- Purified anti-SSRP1
- Antibody type
- Monoclonal
- Antigen
- Recombinant (partial)
- Description
- SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 3E4 monoclonal antibody recognizes human SSRP1 and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- 293E cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SSRP1 Antibody (clone 3E4). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
