Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-90790 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FDAIANIRGE TFFFKGPWFW RLQPSGQLVS PRPARLHRFW EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG GARPLTELGL PPGEEVDAVF SWPQNGKTYL VRGRQYWRYD EAAARPDPGY PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG AHYWRFPKNS IKTEPDAPQP MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQA Positive Samples: A-549, HT-29, Mouse lung, Mouse liver, Rat liver; Cellular Location: Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, extracellular matrix, extracellular space
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.14 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MMP25 in extracts of various cell lines using MMP25 Polyclonal Antibody (Product # PA5-90790) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MMP25 was performed in HeLa cells using MMP25 Polyclonal Antibody (Product # PA5-90790).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MMP25 was performed in L929 cells using MMP25 Polyclonal Antibody (Product # PA5-90790).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MMP25 in paraffin-embedded rat spleen using MMP25 Polyclonal Antibody (Product # PA5-90790) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MMP25 in paraffin-embedded human lung cancer using MMP25 Polyclonal Antibody (Product # PA5-90790) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MMP25 in paraffin-embedded mouse spleen using MMP25 Polyclonal Antibody (Product # PA5-90790) at a dilution of 1:100.
