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Learn100-401-244
antibody from Rockland Immunochemicals, Inc.
Targeting: DIABLO
DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 100-401-244 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-244, RRID:AB_2092781
- Product name
- Anti-Smac/DIABLO (RABBIT) Antibody - 100-401-244
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using anti-Smac detects a 26 kDa band when 1 µg of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 µg of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 µg each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 µM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1
- Validation comment
- Western Blot
