209-401-B49

antibody from Rockland Immunochemicals, Inc.

Targeting: IL32 NK4, TAIF, TAIFb, TAIFd

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

209-401-B49

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#209-401-B49, RRID:AB_1961729

Product name

Anti-Human IL-32A (RABBIT) Antibody - 209-401-B49

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recom-binant human IL-32A (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

Validation comment

Western Blot