PA5-30473

antibody from Invitrogen Antibodies

Targeting: SEC14L2 C22orf6, KIAA1186, KIAA1658, SPF, TAP, TAP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30473

Provider

Invitrogen Antibodies

Product name

SEC14L2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG. Predicted reactivity: Mouse (93%), Rat (94%), Pig (88%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.43 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SEC14L2 Polyclonal Antibody (Product # PA5-30473). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 10% SDS PAGE. SEC14L2 Polyclonal Antibody (Product # PA5-30473) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SEC14L2 Polyclonal Antibody detects SEC14L2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC14L2 protein stained by SEC14L2 Polyclonal Antibody (Product # PA5-30473) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using SEC14L2 (Product # PA5-30473) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.