Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB500-238AF700 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Survivin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Survivin Antibody (60.11) [NB500-238] is specific for the cytoplasmic form of Survivin.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Near infrared dye
- Antigen sequence
The epitope recognized is between a
mino acids 27-67- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Survivin Antibody (60.11) [Alexa Fluor® 700] [NB500-238AF700] - An intracellular stain was performed on U2OS cells with Survivin Antibody (60.11) [Alexa Fluor 700] [NB500-238AF700] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.