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- Antibody Data
- Antigen structure
- References [43]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- AF886 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Survivin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Survivin. This antibody also detects an unidentified ~51 kDa protein band.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.
Mitochondrial ncRNA targeting induces cell cycle arrest and tumor growth inhibition of MDA-MB-231 breast cancer cells through reduction of key cell cycle progression factors.
NGF-Enhanced Vasculogenic Properties of Epithelial Ovarian Cancer Cells Is Reduced by Inhibition of the COX-2/PGE(2) Signaling Axis.
Cloning, expression, and purification of the recombinant pro-apoptotic dominant-negative survivin T34A-C84A protein in Escherichia coli.
Disruption of Wnt/β-Catenin Exerts Antileukemia Activity and Synergizes with FLT3 Inhibition in FLT3-Mutant Acute Myeloid Leukemia.
Radiation Sensitization of Basal Cell and Head and Neck Squamous Cell Carcinoma by the Hedgehog Pathway Inhibitor Vismodegib.
Epigenetic silencing of XAF1 in high-grade gliomas is associated with IDH1 status and improved clinical outcome.
Chromosomal instability induced by increased BIRC5/Survivin levels affects tumorigenicity of glioma cells.
Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study.
Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies.
USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.
STAT3 activation is required for the antiapoptotic effects of prolactin in cervical cancer cells.
Sulindac sulfide inhibits colon cancer cell growth and downregulates specificity protein transcription factors.
Stage-dependent prognostic impact of molecular signatures in clear cell renal cell carcinoma.
Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer.
Survivin expression promotes VEGF-induced tumor angiogenesis via PI3K/Akt enhanced β-catenin/Tcf-Lef dependent transcription.
The pterocarpanquinone LQB-118 induces apoptosis in acute myeloid leukemia cells of distinct molecular subtypes and targets FoxO3a and FoxM1 transcription factors.
Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53.
COX-2-independent effects of celecoxib sensitize lymphoma B cells to TRAIL-mediated apoptosis.
Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair.
Sequence-dependent combination therapy with doxorubicin and a survivin-specific small interfering RNA nanodrug demonstrates efficacy in models of adenocarcinoma.
Azadirone, a limonoid tetranortriterpene, induces death receptors and sensitizes human cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through a p53 protein-independent mechanism: evidence for the role of the ROS-ERK-CHOP-death receptor pathway.
The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study.
Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-κB-dependent manner.
Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis.
Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
Myricetin sensitizes malignant glioma cells to TRAIL-mediated apoptosis by down-regulation of the short isoform of FLIP and bcl-2.
SOX7, down-regulated in colorectal cancer, induces apoptosis and inhibits proliferation of colorectal cancer cells.
Regulation of apoptosis by XIAP ubiquitin-ligase activity.
Cell polarization during monopolar cytokinesis.
Nuclear localization of Survivin renders HeLa tumor cells more sensitive to apoptosis by induction of p53 and Bax.
Survivin in the human hair follicle.
Mitogen-activated protein kinase kinase inhibition enhances nuclear proapoptotic function of p53 in acute myelogenous leukemia cells.
Indirubin-3'-monoxime, a CDK inhibitor induces growth inhibition and apoptosis-independent up-regulation of survivin in transitional cell cancer.
cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis.
Inflammation-associated cell cycle-independent block of apoptosis by survivin in terminally differentiated neutrophils.
Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival.
Localization, dynamics, and function of survivin revealed by expression of functional survivinDsRed fusion proteins in the living cell.
Lin TY, Chan HH, Chen SH, Sarvagalla S, Chen PS, Coumar MS, Cheng SM, Chang YC, Lin CH, Leung E, Cheung CHA
Autophagy 2020 Jul;16(7):1296-1313
Autophagy 2020 Jul;16(7):1296-1313
Mitochondrial ncRNA targeting induces cell cycle arrest and tumor growth inhibition of MDA-MB-231 breast cancer cells through reduction of key cell cycle progression factors.
Fitzpatrick C, Bendek MF, Briones M, Farfán N, Silva VA, Nardocci G, Montecino M, Boland A, Deleuze JF, Villegas J, Villota C, Silva V, Lobos-Gonzalez L, Borgna V, Barrey E, Burzio LO, Burzio VA
Cell death & disease 2019 May 29;10(6):423
Cell death & disease 2019 May 29;10(6):423
NGF-Enhanced Vasculogenic Properties of Epithelial Ovarian Cancer Cells Is Reduced by Inhibition of the COX-2/PGE(2) Signaling Axis.
Garrido MP, Hurtado I, Valenzuela-Valderrama M, Salvatierra R, Hernández A, Vega M, Selman A, Quest AFG, Romero C
Cancers 2019 Dec 7;11(12)
Cancers 2019 Dec 7;11(12)
Cloning, expression, and purification of the recombinant pro-apoptotic dominant-negative survivin T34A-C84A protein in Escherichia coli.
Tsai SL, Chang YC, Sarvagalla S, Wang S, Coumar MS, Cheung CHA
Protein expression and purification 2019 Aug;160:73-83
Protein expression and purification 2019 Aug;160:73-83
Disruption of Wnt/β-Catenin Exerts Antileukemia Activity and Synergizes with FLT3 Inhibition in FLT3-Mutant Acute Myeloid Leukemia.
Jiang X, Mak PY, Mu H, Tao W, Mak DH, Kornblau S, Zhang Q, Ruvolo P, Burks JK, Zhang W, McQueen T, Pan R, Zhou H, Konopleva M, Cortes J, Liu Q, Andreeff M, Carter BZ
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 May 15;24(10):2417-2429
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 May 15;24(10):2417-2429
Radiation Sensitization of Basal Cell and Head and Neck Squamous Cell Carcinoma by the Hedgehog Pathway Inhibitor Vismodegib.
Hehlgans S, Booms P, Güllülü Ö, Sader R, Rödel C, Balermpas P, Rödel F, Ghanaati S
International journal of molecular sciences 2018 Aug 23;19(9)
International journal of molecular sciences 2018 Aug 23;19(9)
Epigenetic silencing of XAF1 in high-grade gliomas is associated with IDH1 status and improved clinical outcome.
Reich TR, Switzeny OJ, Renovanz M, Sommer C, Kaina B, Christmann M, Tomicic MT
Oncotarget 2017 Feb 28;8(9):15071-15084
Oncotarget 2017 Feb 28;8(9):15071-15084
Chromosomal instability induced by increased BIRC5/Survivin levels affects tumorigenicity of glioma cells.
Conde M, Michen S, Wiedemuth R, Klink B, Schröck E, Schackert G, Temme A
BMC cancer 2017 Dec 28;17(1):889
BMC cancer 2017 Dec 28;17(1):889
Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study.
Lin KY, Cheng SM, Tsai SL, Tsai JY, Lin CH, Cheung CH
OncoTargets and therapy 2016;9:2601-13
OncoTargets and therapy 2016;9:2601-13
Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies.
Mędra A, Witkowska M, Majchrzak A, Cebula-Obrzut B, Bonner MY, Robak T, Arbiser JL, Smolewski P
Molecules (Basel, Switzerland) 2016 Jul 30;21(8)
Molecules (Basel, Switzerland) 2016 Jul 30;21(8)
USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.
Engel K, Rudelius M, Slawska J, Jacobs L, Ahangarian Abhari B, Altmann B, Kurutz J, Rathakrishnan A, Fernández-Sáiz V, Brunner A, Targosz BS, Loewecke F, Gloeckner CJ, Ueffing M, Fulda S, Pfreundschuh M, Trümper L, Klapper W, Keller U, Jost PJ, Rosenwald A, Peschel C, Bassermann F
EMBO molecular medicine 2016 Aug;8(8):851-62
EMBO molecular medicine 2016 Aug;8(8):851-62
STAT3 activation is required for the antiapoptotic effects of prolactin in cervical cancer cells.
Ramírez de Arellano A, Lopez-Pulido EI, Martínez-Neri PA, Estrada Chávez C, González Lucano R, Fafutis-Morris M, Aguilar-Lemarroy A, Muñoz-Valle JF, Pereira-Suárez AL
Cancer cell international 2015;15:83
Cancer cell international 2015;15:83
Sulindac sulfide inhibits colon cancer cell growth and downregulates specificity protein transcription factors.
Li X, Pathi SS, Safe S
BMC cancer 2015 Dec 16;15:974
BMC cancer 2015 Dec 16;15:974
Stage-dependent prognostic impact of molecular signatures in clear cell renal cell carcinoma.
Weber T, Meinhardt M, Zastrow S, Wienke A, Erdmann K, Hofmann J, Fuessel S, Wirth MP
OncoTargets and therapy 2014;7:645-54
OncoTargets and therapy 2014;7:645-54
Protein kinase C ζ regulates survivin expression and inhibits apoptosis in colon cancer.
Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N
International journal of oncology 2014 Sep;45(3):1043-50
International journal of oncology 2014 Sep;45(3):1043-50
Survivin expression promotes VEGF-induced tumor angiogenesis via PI3K/Akt enhanced β-catenin/Tcf-Lef dependent transcription.
Fernández JG, Rodríguez DA, Valenzuela M, Calderon C, Urzúa U, Munroe D, Rosas C, Lemus D, Díaz N, Wright MC, Leyton L, Tapia JC, Quest AF
Molecular cancer 2014 Sep 9;13:209
Molecular cancer 2014 Sep 9;13:209
The pterocarpanquinone LQB-118 induces apoptosis in acute myeloid leukemia cells of distinct molecular subtypes and targets FoxO3a and FoxM1 transcription factors.
Nestal de Moraes G, Castro CP, Salustiano EJ, Dumas ML, Costas F, Lam EW, Costa PR, Maia RC
International journal of oncology 2014 Nov;45(5):1949-58
International journal of oncology 2014 Nov;45(5):1949-58
Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53.
Wiedemuth R, Klink B, Töpfer K, Schröck E, Schackert G, Tatsuka M, Temme A
Molecular cancer 2014 May 9;13:107
Molecular cancer 2014 May 9;13:107
COX-2-independent effects of celecoxib sensitize lymphoma B cells to TRAIL-mediated apoptosis.
Gallouet AS, Travert M, Bresson-Bepoldin L, Guilloton F, Pangault C, Caulet-Maugendre S, Lamy T, Tarte K, Guillaudeux T
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 May 15;20(10):2663-73
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 May 15;20(10):2663-73
Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair.
Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J, Zhang X, Dai S, Sun X
Journal of hematology & oncology 2014 Aug 20;7:62
Journal of hematology & oncology 2014 Aug 20;7:62
Sequence-dependent combination therapy with doxorubicin and a survivin-specific small interfering RNA nanodrug demonstrates efficacy in models of adenocarcinoma.
Ghosh SK, Yigit MV, Uchida M, Ross AW, Barteneva N, Moore A, Medarova Z
International journal of cancer 2014 Apr 1;134(7):1758-66
International journal of cancer 2014 Apr 1;134(7):1758-66
Azadirone, a limonoid tetranortriterpene, induces death receptors and sensitizes human cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through a p53 protein-independent mechanism: evidence for the role of the ROS-ERK-CHOP-death receptor pathway.
Gupta SC, Francis SK, Nair MS, Mo YY, Aggarwal BB
The Journal of biological chemistry 2013 Nov 8;288(45):32343-56
The Journal of biological chemistry 2013 Nov 8;288(45):32343-56
The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
Hage C, Rausch V, Giese N, Giese T, Schönsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
Cell death & disease 2013 May 9;4:e627
Cell death & disease 2013 May 9;4:e627
Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study.
Muñoz-Repeto I, García MJ, Kamieniak M, Ramón Y Cajal T, Domingo S, Cazorla A, García Donas J, Hernando Polo S, García Sagredo JM, Hernández E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benítez J
Histology and histopathology 2013 Jan;28(1):133-44
Histology and histopathology 2013 Jan;28(1):133-44
Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-κB-dependent manner.
Wagner L, Marschall V, Karl S, Cristofanon S, Zobel K, Deshayes K, Vucic D, Debatin KM, Fulda S
Oncogene 2013 Feb 21;32(8):988-97
Oncogene 2013 Feb 21;32(8):988-97
Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
Engesæter B, Engebraaten O, Flørenes VA, Mælandsmo GM
PloS one 2012;7(9):e45492
PloS one 2012;7(9):e45492
RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis.
Löder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S
Leukemia 2012 May;26(5):1020-9
Leukemia 2012 May;26(5):1020-9
Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
Toosi S, Habib N, Torres G, Reynolds SR, Bystryn JC
The Journal of investigative dermatology 2011 Nov;131(11):2327-9
The Journal of investigative dermatology 2011 Nov;131(11):2327-9
Myricetin sensitizes malignant glioma cells to TRAIL-mediated apoptosis by down-regulation of the short isoform of FLIP and bcl-2.
Siegelin MD, Gaiser T, Habel A, Siegelin Y
Cancer letters 2009 Oct 8;283(2):230-8
Cancer letters 2009 Oct 8;283(2):230-8
SOX7, down-regulated in colorectal cancer, induces apoptosis and inhibits proliferation of colorectal cancer cells.
Zhang Y, Huang S, Dong W, Li L, Feng Y, Pan L, Han Z, Wang X, Ren G, Su D, Huang B, Lu J
Cancer letters 2009 May 8;277(1):29-37
Cancer letters 2009 May 8;277(1):29-37
Regulation of apoptosis by XIAP ubiquitin-ligase activity.
Schile AJ, García-Fernández M, Steller H
Genes & development 2008 Aug 15;22(16):2256-66
Genes & development 2008 Aug 15;22(16):2256-66
Cell polarization during monopolar cytokinesis.
Hu CK, Coughlin M, Field CM, Mitchison TJ
The Journal of cell biology 2008 Apr 21;181(2):195-202
The Journal of cell biology 2008 Apr 21;181(2):195-202
Nuclear localization of Survivin renders HeLa tumor cells more sensitive to apoptosis by induction of p53 and Bax.
Temme A, Rodriguez JA, Hendruschk S, Günes S, Weigle B, Schäkel K, Schmitz M, Bachmann M, Schackert G, Rieber EP
Cancer letters 2007 Jun 8;250(2):177-93
Cancer letters 2007 Jun 8;250(2):177-93
Survivin in the human hair follicle.
Botchkareva NV, Kahn M, Ahluwalia G, Shander D
The Journal of investigative dermatology 2007 Feb;127(2):479-82
The Journal of investigative dermatology 2007 Feb;127(2):479-82
Mitogen-activated protein kinase kinase inhibition enhances nuclear proapoptotic function of p53 in acute myelogenous leukemia cells.
Kojima K, Konopleva M, Samudio IJ, Ruvolo V, Andreeff M
Cancer research 2007 Apr 1;67(7):3210-9
Cancer research 2007 Apr 1;67(7):3210-9
Indirubin-3'-monoxime, a CDK inhibitor induces growth inhibition and apoptosis-independent up-regulation of survivin in transitional cell cancer.
Perabo FG, Frössler C, Landwehrs G, Schmidt DH, von Rücker A, Wirger A, Müller SC
Anticancer research 2006 May-Jun;26(3A):2129-35
Anticancer research 2006 May-Jun;26(3A):2129-35
cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
European journal of immunology 2006 Jul;36(7):1975-84
European journal of immunology 2006 Jul;36(7):1975-84
Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis.
McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR
Experimental cell research 2005 Jan 1;302(1):69-82
Experimental cell research 2005 Jan 1;302(1):69-82
Inflammation-associated cell cycle-independent block of apoptosis by survivin in terminally differentiated neutrophils.
Altznauer F, Martinelli S, Yousefi S, Thürig C, Schmid I, Conway EM, Schöni MH, Vogt P, Mueller C, Fey MF, Zangemeister-Wittke U, Simon HU
The Journal of experimental medicine 2004 May 17;199(10):1343-54
The Journal of experimental medicine 2004 May 17;199(10):1343-54
Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
Li CQ, Robles AI, Hanigan CL, Hofseth LJ, Trudel LJ, Harris CC, Wogan GN
Cancer research 2004 May 1;64(9):3022-9
Cancer research 2004 May 1;64(9):3022-9
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H
Cell 2004 Jul 23;118(2):187-202
Cell 2004 Jul 23;118(2):187-202
Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival.
Kappler M, Kotzsch M, Bartel F, Füssel S, Lautenschläger C, Schmidt U, Würl P, Bache M, Schmidt H, Taubert H, Meye A
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Mar;9(3):1098-104
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Mar;9(3):1098-104
Localization, dynamics, and function of survivin revealed by expression of functional survivinDsRed fusion proteins in the living cell.
Temme A, Rieger M, Reber F, Lindemann D, Weigle B, Diestelkoetter-Bachert P, Ehninger G, Tatsuka M, Terada Y, Rieber EP
Molecular biology of the cell 2003 Jan;14(1):78-92
Molecular biology of the cell 2003 Jan;14(1):78-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Survivin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Survivin at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Survivin by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Survivin in Human Prostate Cancer Tissue. Survivin was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 10 µg/mL Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Survivin in Human Breast Cancer Tissue. Survivin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
