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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AP09149PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09149PU-N, RRID:AB_2035652
- Product name
- anti IAP4 / BIRC5
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near the carboxy terminus of Human Survivin protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using IgG Fraction of anti-Survivin antibody shows detection of a single band at ~17 kDa corresponding to Survivin in MOLT4 cell lysates in the absence (lane A) and presence (lane B) of blocking peptide. Primary antibody was used at 1 µg/ml.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical staining using IgG Fraction of anti-Survivin antibody at 10 µg/ml shows detection of Survivin in MOLT4 cells. Cells were fixed in 4% paraformaldehyde.
