Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R30128 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Survivin Antibody / BIRC5
- Antibody type
- Polyclonal
- Description
- This highly specific Survivin antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Survivin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Survivin antibody and Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) Jurkat and 3) 293T cell lysate with Survivin antibody. Predicted molecular weight ~16 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colon cancer tissue with Survivin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.