A00379

antibody from Boster Biological Technology

Targeting: BIRC5 API4, EPR-1, survivin

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00379

Provider

Boster Biological Technology

Product name

Anti-Survivin/BIRC5 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for Survivin/BIRC5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ELISA. Reactive species: Human, Mouse, Rat. Survivin/BIRC5 information: Molecular Weight: 16389 MW; Subcellular Localization: Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export; Tissue Specificity: Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Isolation of an "Early" Transit Amplifying Keratinocyte Population in Human Epidermis: A Role for the Low Affinity Neurotrophin Receptor CD271.

Lotti R, Palazzo E, Quadri M, Dumas M, Schnebert S, Biondini D, Bianchini MA, Nizard C, Pincelli C, Marconi A
Stem cells (Dayton, Ohio) 2022 Dec 31;40(12):1149-1161

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Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells.

Wang Y, Li M, Diao R, Tung B, Zhang D, Li Y
Oncotarget 2017 Aug 1;8(31):51652-51662

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Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway.

Huang KF, Huang XP, Xiao GQ, Yang HY, Lin JS, Diao Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2014 May;68(4):455-61

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Antisurvivin oligonucleotides inhibit growth and induce apoptosis in human medullary thyroid carcinoma cells.

Du ZX, Zhang HY, Gao DX, Wang HQ, Li YJ, Liu GL
Experimental & molecular medicine 2006 Jun 30;38(3):230-40

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Survivin using anti-Survivin antibody (A00379). Survivin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Survivin Antibody (A00379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image