PAB11271

antibody from Abnova Corporation

Targeting: BIRC5 API4, EPR-1, survivin

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB11271

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB11271, RRID:AB_1671610

Product name

BIRC5 polyclonal antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to C-terminus of human BIRC5.

Reactivity

Human

Host

Rabbit

Vial size

100 μg

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation.

Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T
Proceedings of the National Academy of Sciences of the United States of America 1999 Feb 16;96(4):1457-62

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Control of apoptosis and mitotic spindle checkpoint by survivin.

Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC
Nature 1998 Dec 10;396(6711):580-4

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A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma.

Ambrosini G, Adida C, Altieri DC
Nature medicine 1997 Aug;3(8):917-21

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using BIRC5 polyclonal antibody (Cat # PAB11271) shows detection of a single band at ~17 kDa corresponding to BIRC5 in MOLT 4 cell lysates in the absence (lane A) and presence (lane B) of blocking peptide.The primary antibody was used at 1 ug/mL .

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemical staining using BIRC5 polyclonal antibody (Cat # PAB11271) at 10 ug/mL shows detection of BIRC5 in MOLT 4 cells. Cells were fixed in 4% paraformaldehyde.

Validation comment

Immunocytochemistry