Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M00379-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Survivin Birc5 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for Survivin/Birc5 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IHC, WB. Reactive species: Mouse, Rat Survivin/Birc5 information: Molecular Weight: 16298 MW; Subcellular Localization: Cytoplasm . Nucleus . Chromosome. Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Midbody . Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (By similarity).
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- DGD-2
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells.
Increased survivin expression and its association with clinical parameters of congenital choledochal cysts.
Significance of survivin, caspase-3, and VEGF expression in thyroid carcinoma.
Wang Y, Li M, Diao R, Tung B, Zhang D, Li Y
Oncotarget 2017 Aug 1;8(31):51652-51662
Oncotarget 2017 Aug 1;8(31):51652-51662
Increased survivin expression and its association with clinical parameters of congenital choledochal cysts.
Wu ZG, Li GX, Wang B, Chen ZM, Feng Q
Genetics and molecular research : GMR 2016 Jun 20;15(2)
Genetics and molecular research : GMR 2016 Jun 20;15(2)
Significance of survivin, caspase-3, and VEGF expression in thyroid carcinoma.
Zhang HY, Meng X, Du ZX, Fang CQ, Liu GL, Wang HQ, Deng WW
Clinical and experimental medicine 2009 Sep;9(3):207-13
Clinical and experimental medicine 2009 Sep;9(3):207-13
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Survivin Antibody(M00379-2)Birc5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Birc5 Antibody (M00379-2)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image