Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-16838 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Survivin Monoclonal Antibody (60.11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Suggested positive control: Hela whole cell extract, antigen standard for BIRC5 (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 60.11
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Survivin detected in rat aorta smooth muscle cell lysate using Product # MA1-16838.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Survivin in 5 µg per lane cells transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA. Samples were incubated in Survivin monoclonal antibody (Product # MA1-16838). Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates were separated by SDS-PAGE and immunoblotted. Note: theoretical molecular weight for Survivin is 16 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Survivin in rat aorta smooth muscle cell lysate. Samples were incubated in Survivin monoclonal antibody (Product # MA1-16838). Theoretical molecular weight: 16 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Survivin Monoclonal Antibody (60.11) (Product # MA1-16838) and a 17 kDa band corresponding to Survivin was observed across cell lines tested and decreased upon LY294002 treatment in SK-BR-3. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), A549 (Lane 3), K-562 (Lane 4), A-431 (Lane 5), SK-BR-3 (Lane 6) and SK-BR-3 treated with LY294002 (10uM for 24 hours) (Lane 7) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells stained MA1-16838 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Survivin in formalin-fixed paraffin-embedded ovarian cancer tissue. Samples were incubated in Survivin monoclonal antibody (Product # MA1-16838).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FACS staining of NTERA-2 cells using Product # MA1-16838 at a 1:2000 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Survivin in A549 cells. Samples were incubated in Survivin monoclonal antibody (Product # MA1-16838) and a matched isotype control using a dilution of 1 µg/mL for 30 minutes at room temperature followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.
