- PA5-16859 - Invitrogen Antibodies BIRC5 antibody

Submitted references Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice.

Abdin AA, Soliman NA, Saied EM
Pharmacological reports : PR 2014 Dec;66(6):1114-21

Assessing DcR3 expression in relation to survivin and other prognostic factors in B cell non-Hodgkin's lymphoma.

Bedewy AM, Elgammal MM, Bedewy MM, El-Maghraby SM
Annals of hematology 2013 Oct;92(10):1359-67

Expression of nuclear factor kappa B and survivin in psoriasis.

Gunduz K, Temiz P, Gencoglan G, Inanir I, Catalkaya A
ISRN dermatology 2012;2012:257059

Survivin expression in patients with newly diagnosed nodal diffuse large B cell lymphoma (DLBCL).

Markovic O, Marisavljevic D, Cemerikic-Martinovic V, Martinovic T, Filipovic B, Stanisavljevic D, Zivković R, Hajder J, Stanisavljevic N, Mihaljevic B
Medical oncology (Northwood, London, England) 2012 Dec;29(5):3515-21

Weapons ovarian epithelial tumors may use in immune escape: an immunohistochemical correlational study.

Hasby EA
Pathology oncology research : POR 2012 Apr;18(2):509-18

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Survivin (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Survivin polyclonal antibody (Product # PA5-16859) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Survivin was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Survivin Rabbit Polyclonal antibody (Product # PA5-16859 ) at 5µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature. Progression of cells through subsequent phases of the cell cycle results in differential localization of Survivin in centromeres, spindle midzone and midbody of two daughter cells. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Survivin was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Survivin Rabbit Polyclonal antibody (Product # PA5-16859) at 5 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human pancreas stained with Survivin using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of islet cells.

PA5-16859

Antibody data