NB500-238

antibody from Novus Biologicals

Targeting: BIRC5 API4, EPR-1, survivin

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

NB500-238

Provider

Novus Biologicals

Proper citation

Novus Cat#NB500-238, RRID:AB_10001297

Product name

Mouse Monoclonal Survivin Antibody

Antibody type

Monoclonal

Description

Protein G purified. Survivin Antibody (60.11) [NB500-238] is specific for the cytoplasmic form of Survivin.

Reactivity

Human, Mouse, Rat

Host

Mouse

Antigen sequence

The epitope recognized is between a
mino acids 27-67


Isotype

IgG

Vial size

0.1 ml

Concentration

0.9 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Improvement of Inflammation through Antioxidant Pathway of Gardeniae Fructus 50% EtOH Extract (GE) from Acute Reflux Esophagitis Rats.

Kim SH, Shin MR, Lee AR, Seo BI, Park HJ, Roh SS
BioMed research international 2020;2020:4826176

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BIRC5 Expression is Regulated in Uterine Epithelium During the Estrous Cycle.

Cho M, Lee OH, Chang EM, Lee S, Moon S, Lee J, Park H, Park KH, Park C, Hong K, Choi Y
Genes 2020 Mar 6;11(3)

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HDAC inhibitors restore BRAF-inhibitor sensitivity by altering PI3K and survival signalling in a subset of melanoma.

Gallagher SJ, Gunatilake D, Beaumont KA, Sharp DM, Tiffen JC, Heinemann A, Weninger W, Haass NK, Wilmott JS, Madore J, Ferguson PM, Rizos H, Hersey P
International journal of cancer 2018 May 1;142(9):1926-1937

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Survivin Monoclonal Antibodies Detect Survivin Cell Surface Expression and Inhibit Tumor Growth In Vivo.

Fenstermaker RA, Figel SA, Qiu J, Barone TA, Dharma SS, Winograd EK, Galbo PM, Wiltsie LM, Ciesielski MJ
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Jun 1;24(11):2642-2652

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CBP/β-Catenin/FOXM1 Is a Novel Therapeutic Target in Triple Negative Breast Cancer.

Ring A, Nguyen C, Smbatyan G, Tripathy D, Yu M, Press M, Kahn M, Lang JE
Cancers 2018 Dec 19;10(12)

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Subcellular Localization of Survivin Determines Its Function in Cardiomyocytes.

Tsang TJ, Hsueh YC, Wei EI, Lundy DJ, Cheng B, Chen YT, Wang SS, Hsieh PCH
Theranostics 2017;7(18):4577-4590

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Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency.

Dheekollu J, Malecka K, Wiedmer A, Delecluse HJ, Chiang AK, Altieri DC, Messick TE, Lieberman PM
Oncotarget 2017 Jan 31;8(5):7248-7264

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Circulating CD9+/GFAP+/survivin+ exosomes in malignant glioma patients following survivin vaccination.

Galbo PM Jr, Ciesielski MJ, Figel S, Maguire O, Qiu J, Wiltsie L, Minderman H, Fenstermaker RA
Oncotarget 2017 Dec 29;8(70):114722-114735

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Correlation of survivin and B-cell lymphoma 2 expression with pathological malignancy and anti-apoptotic properties of glial cell tumors.

Bae IS, Kim CH, Kim JM, Cheong JH, Ryu JI, Han MH
Biomedical reports 2017 Apr;6(4):396-400

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Cell death in response to antimetabolites directed at ribonucleotide reductase and thymidylate synthase.

Asuncion Valenzuela MM, Castro I, Gonda A, Diaz Osterman CJ, Jutzy JM, Aspe JR, Khan S, Neidigh JW, Wall NR
OncoTargets and therapy 2015;8:495-507

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microRNA-mediated survivin control of pluripotency.

Kapinas K, Kim H, Mandeville M, Martin-Buley LA, Croce CM, Lian JB, van Wijnen AJ, Stein JL, Altieri DC, Stein GS
Journal of cellular physiology 2015 Jan;230(1):63-70

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Survivin as a potential mediator to support autoreactive cell survival in myasthenia gravis: a human and animal model study.

Kusner LL, Ciesielski MJ, Marx A, Kaminski HJ, Fenstermaker RA
PloS one 2014;9(7):e102231

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High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays.

Rho JH, Lampe PD
Journal of proteome research 2013 May 3;12(5):2311-20

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Plasma-derived exosomal survivin, a plausible biomarker for early detection of prostate cancer.

Khan S, Jutzy JM, Valenzuela MM, Turay D, Aspe JR, Ashok A, Mirshahidi S, Mercola D, Lilly MB, Wall NR
PloS one 2012;7(10):e46737

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A novel role for survivin in erythroblast enucleation.

Keerthivasan G, Liu H, Gump JM, Dowdy SF, Wickrema A, Crispino JD
Haematologica 2012 Oct;97(10):1471-9

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DNA and adenovirus tumor vaccine expressing truncated survivin generates specific immune responses and anti-tumor effects in a murine melanoma model.

Zhang H, Wang Y, Liu C, Zhang L, Xia Q, Zhang Y, Wu J, Jiang C, Chen Y, Wu Y, Zha X, Yu X, Kong W
Cancer immunology, immunotherapy : CII 2012 Oct;61(10):1857-67

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Mitochondrial heat shock protein-90 modulates vascular smooth muscle cell survival and the vascular injury response in vivo.

Hoel AW, Yu P, Nguyen KP, Sui X, Plescia J, Altieri DC, Conte MS
The American journal of pathology 2012 Oct;181(4):1151-7

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Increased levels of survivin, via association with heat shock protein 90, in mucosal T cells from patients with Crohn's disease.

de Souza HS, West GA, Rebert N, de la Motte C, Drazba J, Fiocchi C
Gastroenterology 2012 Oct;143(4):1017-26.e9

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Chk2 phosphorylation of survivin-DeltaEx3 contributes to a DNA damage-sensing checkpoint in cancer.

Lopergolo A, Tavecchio M, Lisanti S, Ghosh JC, Dohi T, Faversani A, Vaira V, Bosari S, Tanigawa N, Delia D, Kossenkov AV, Showe LC, Altieri DC
Cancer research 2012 Jul 1;72(13):3251-9

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Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo.

Sakao K, Desineni S, Hahm ER, Singh SV
The Prostate 2012 Jul 1;72(10):1104-16

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Sustained proliferation and resistance to apoptosis after a cytotoxic insult are early alterations in rat colon carcinogenesis.

Femia AP, Salvianti F, Luceri C, Dolara P, Salvadori M, Pinzani P, Caderni G
International journal of cancer 2012 Aug 1;131(3):529-36

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The oncofetal gene survivin promotes cell proliferation and survival in primary human osteoblastic cells.

Lechler P, Schaumburger J, Köck FX, Balakrishnan S, Doukas S, Prantl L, Grifka J
Calcified tissue international 2011 Sep;89(3):211-20

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The integration of a Stat3 specific peptide aptamer into the thioredoxin scaffold protein strongly enhances its inhibitory potency.

Schöneberger H, Weiss A, Brill B, Delis N, Borghouts C, Groner B
Hormone molecular biology and clinical investigation 2011 Mar 1;5(1):1-9

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The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro.

Lechler P, Balakrishnan S, Schaumburger J, Grässel S, Baier C, Grifka J, Straub RH, Renkawitz T
BMC musculoskeletal disorders 2011 Jul 5;12:150

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The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro.

Lechler P, Renkawitz T, Campean V, Balakrishnan S, Tingart M, Grifka J, Schaumburger J
BMC cancer 2011 Apr 2;11:120

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Expression analysis suggests a potential cytoprotective role of Birc5 in the inner ear.

Habtemichael N, Heinrich UR, Knauer SK, Schmidtmann I, Bier C, Docter D, Brochhausen C, Helling K, Brieger J, Stauber RH, Mann WJ
Molecular and cellular neurosciences 2010 Nov;45(3):297-305

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An otoprotective role for the apoptosis inhibitor protein survivin.

Knauer SK, Heinrich UR, Bier C, Habtemichael N, Docter D, Helling K, Mann WJ, Stauber RH
Cell death & disease 2010 Jul 1;1:e51

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Cloning and functional characterization of the guinea pig apoptosis inhibitor protein Survivin.

Habtemichael N, Wünsch D, Bier C, Tillmann S, Unruhe B, Frauenknecht K, Heinrich UR, Mann WJ, Stauber RH, Knauer SK
Gene 2010 Dec 1;469(1-2):9-17

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Abnormal cytokinesis after X-irradiation in tumor cells that override the G2 DNA damage checkpoint.

Huang H, Fletcher L, Beeharry N, Daniel R, Kao G, Yen TJ, Muschel RJ
Cancer research 2008 May 15;68(10):3724-32

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Survivin determines cardiac function by controlling total cardiomyocyte number.

Levkau B, Schäfers M, Wohlschlaeger J, von Wnuck Lipinski K, Keul P, Hermann S, Kawaguchi N, Kirchhof P, Fabritz L, Stypmann J, Stegger L, Flögel U, Schrader J, Fischer JW, Hsieh P, Ou YL, Mehrhof F, Tiemann K, Ghanem A, Matus M, Neumann J, Heusch G, Schmid KW, Conway EM, Baba HA
Circulation 2008 Mar 25;117(12):1583-93

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Survival advantage of EBV-associated gastric carcinoma: survivin up-regulation by viral latent membrane protein 2A.

Hino R, Uozaki H, Inoue Y, Shintani Y, Ushiku T, Sakatani T, Takada K, Fukayama M
Cancer research 2008 Mar 1;68(5):1427-35

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Mitochondrial targeting of adenomatous polyposis coli protein is stimulated by truncating cancer mutations: regulation of Bcl-2 and implications for cell survival.

Brocardo M, Lei Y, Tighe A, Taylor SS, Mok MT, Henderson BR
The Journal of biological chemistry 2008 Feb 29;283(9):5950-9

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The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney.

Lechler P, Wu X, Bernhardt W, Campean V, Gastiger S, Hackenbeck T, Klanke B, Weidemann A, Warnecke C, Amann K, Engehausen D, Willam C, Eckardt KU, Rödel F, Wiesener MS
The American journal of pathology 2007 Nov;171(5):1483-98

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Expression of survivin and its splice variants survivin-2B and survivin-DeltaEx3 in breast cancer.

Ryan B, O'Donovan N, Browne B, O'Shea C, Crown J, Hill AD, McDermott E, O'Higgins N, Duffy MJ
British journal of cancer 2005 Jan 17;92(1):120-4

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Inhibition of survivin expression suppresses the growth of aggressive non-Hodgkin's lymphoma.

Ansell SM, Arendt BK, Grote DM, Jelinek DF, Novak AJ, Wellik LE, Remstein ED, Bennett CF, Fielding A
Leukemia 2004 Mar;18(3):616-23

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Promyelocytic leukemia protein 4 induces apoptosis by inhibition of survivin expression.

Xu ZX, Zhao RX, Ding T, Tran TT, Zhang W, Pandolfi PP, Chang KS
The Journal of biological chemistry 2004 Jan 16;279(3):1838-44

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Expression of survivin, a novel inhibitor of apoptosis, in superficial transitional cell carcinoma of the bladder.

Ku JH, Kwak C, Lee HS, Park HK, Lee E, Lee SE
The Journal of urology 2004 Feb;171(2 Pt 1):631-5

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Survivin expression in mouse skin prevents papilloma regression and promotes chemical-induced tumor progression.

Allen SM, Florell SR, Hanks AN, Alexander A, Diedrich MJ, Altieri DC, Grossman D
Cancer research 2003 Feb 1;63(3):567-72

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Differential expression of intracellular apoptotic signaling molecules in tumor and tumor-infiltrating lymphocytes during development of invasion and/or metastasis of gastric carcinoma.

Koyama S
Digestive diseases and sciences 2003 Dec;48(12):2290-300

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Differential expression of intracellular apoptotic signaling molecules in tumor and tumor-infiltrating lymphocytes during development of invasion and/or metastasis of gastric carcinoma.

Koyama S
Digestive diseases and sciences 2003 Dec;48(12):2290-300

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Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function.

Fortugno P, Wall NR, Giodini A, O'Connor DS, Plescia J, Padgett KM, Tognin S, Marchisio PC, Altieri DC
Journal of cell science 2002 Feb 1;115(Pt 3):575-85

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Anti-apoptotic protein survivin plays a significant role in tubular morphogenesis of human coronary arteriolar endothelial cells by hypoxic preconditioning.

Zhu L, Fukuda S, Cordis G, Das DK, Maulik N
FEBS letters 2001 Nov 23;508(3):369-74

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Anti-apoptotic protein survivin plays a significant role in tubular morphogenesis of human coronary arteriolar endothelial cells by hypoxic preconditioning.

Zhu L, Fukuda S, Cordis G, Das DK, Maulik N
FEBS letters 2001 Nov 23;508(3):369-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Survivin Antibody (60.11) [NB500-238] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY Survivin cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Survivin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Survivin Antibody (60.11) [NB500-238] - Survivin detection in 30ug of HeLa whole cell extract using [NB500-238] at 1ug/ml. Detected at predicted molecular weight of 16 kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Survivin Antibody (60.11) [NB500-238] - Detected in rat aorta smooth muscle cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-238] - Staining of ovary cancer.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Survivin Antibody (60.11) [NB500-238] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Survivin Antibody (60.11) [NB500-238] - Staining of ovary cancer. Image using the Unpurified format of this antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on Daudi cells with Survivin Antibody (60.11)[NB500-238] (blue) and a matched isotype control, Mouse IgG2a Kappa Light Chain Isotype Control (MG2a-53) [NB600-986] (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on A549 cells with Survivin Antibody (60.11) [NB500-238] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Unpurified form of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on HeLa cells with Survivin Antibody [60.11] NB500-238AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on U2OS cells with Survivin Antibody (60.11) [NB500-238AF700] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.