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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- NBP2-33230 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Caspase-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Caspase-1 Antibody (14F468) - Azide and BSA Free [NBP2-33230] - Analysis in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase-1 Antibody (14F468) - Azide and BSA Free [NBP2-33230] - Analysis in human HeLa (A) and mouse NIH3T3 lysate probed with Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml, respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) - Azide and BSA Free [NBP2-33230] - Analysis in a section of normal lung from human using 5 ug/ml concentration. In this representative lung section, different type of cells including pseudostratified columnar epithelium of bronchiole and the simple squamous epithelium of alveoli may be seen to develop immunoreactivity for Caspase 1. [10X Magnification]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) - Azide and BSA Free [NBP2-33230] - Analysis in a section of normal human colon using 5 ug/ml concentration. Distinct cytoplasmic staining along with some nuclear positivity was observed in crypts/mucosa, and staining was found to be more intense in the absorptive columnar epithelial cells. [10X Magnification]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) - Azide and BSA Free [NBP2-33230] - Analysis in adenocarcinoma of the rectum (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) - Azide and BSA Free [NBP2-33230] - Analysis in a section of normal skin from human using 5 ug/ml concentration. Strong cytoplasmic/nuclear staining developed in all the epidermal cells, blood vessels and some cells of the dermal connective tissues layer. [10X Magnification]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) - Azide and BSA Free [NBP2-33230] - Analysis in a section of human ovarian cancer using 5 ug/ml concentration.
