AF6215

antibody from Novus Biologicals

Targeting: CASP1 ICE, IL1BC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF6215

Provider

Novus Biologicals

Product name

Goat Polyclonal Caspase-1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Caspase-1 in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice.

Lê VB, Dubois J, Couture C, Cavanagh MH, Uyar O, Pizzorno A, Rosa-Calatrava M, Hamelin MÈ, Boivin G
PLoS pathogens 2019 Apr;15(4):e1007689

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Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.

Kortmann J, Brubaker SW, Monack DM
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 1;195(3):815-9

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Staphylococcus aureus activates the NLRP3 inflammasome in human and rat conjunctival goblet cells.

McGilligan VE, Gregory-Ksander MS, Li D, Moore JE, Hodges RR, Gilmore MS, Moore TC, Dartt DA
PloS one 2013;8(9):e74010

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Caspase-1 by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Caspase-1 at approximately 51 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Caspase-1 by Western Blot. Detection of Human Caspase-1 by Western Blot.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Caspase‑1 by Simple WesternTM. Detection of Human Caspase‑1 by Simple WesternTM.