GTX62815

antibody from GeneTex

Targeting: CASP1 ICE, IL1BC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

GTX62815

Provider

GeneTex

Product name

Caspase 1 antibody [EPR4321]

Antibody type

Monoclonal

Antigen

Recombinant fragment corresponding to C-terminus of human Caspase-1.

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

References

Submitted references

Modulation of Macrophage Phenotype by Biodegradable Polyurethane Nanoparticles: Possible Relation between Macrophage Polarization and Immune Response of Nanoparticles.

Huang YJ, Hung KC, Hung HS, Hsu SH
ACS applied materials & interfaces 2018 Jun 13;10(23):19436-19448

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of lysates from (A) THP-1, (B) THP-1+TPA+LPS, (C) Jurkat, (D) C6 and (E) NIH/3T3 cells using Caspase 1 antibody [EPR4321].

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of lysates from (1) untreated THP-1, (2) TPA and LPS treated THP-1, (3) Jurkat, (4) C6 and (5) NIH3T3 cells (10 ?g per lane) using Caspase 1 antibody [EPR4321] at a dilution of 1:1,000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of lysates from (A) THP-1, (B) THP-1+TPA+LPS, (C) Jurkat, (D) C6 and (E) NIH/3T3 cells using Caspase 1 antibody [EPR4321].

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of (1) THP-1, (2) HeLa and (3) Jurkat lysates (10 ?g per lane) using Caspase 1 antibody [EPR4321] at a dilution of 1:10,000.

Validation comment

WB