- PA5-87536 - Invitrogen Antibodies CASP1 antibody

Submitted references IP-Se-06, a Selenylated Imidazo[1,2-a]pyridine, Modulates Intracellular Redox State and Causes Akt/mTOR/HIF-1α and MAPK Signaling Inhibition, Promoting Antiproliferative Effect and Apoptosis in Glioblastoma Cells.

Dos Santos DC, Rafique J, Saba S, Grinevicius VMAS, Filho DW, Zamoner A, Braga AL, Pedrosa RC, Ourique F
Oxidative medicine and cellular longevity 2022;2022:3710449

Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.

Chang ZY, Liu HM, Leu YL, Hsu CH, Lee TY
International journal of molecular sciences 2022 Feb 28;23(5)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Caspase 1 was performed in Raw264.7 cells using Caspase 1 Polyclonal Antibody (Product # PA5-87536) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Caspase 1 was performed in Raw264.7 cells using Caspase 1 Polyclonal Antibody (Product # PA5-87536) at a dilution of 1:200. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation analysis of Caspase 1 was performed in 200 µg extracts of THP-1 cells using Caspase 1 Polyclonal Antibody (Product # PA5-87536). Western blot was performed from the immunoprecipitate using Caspase 1 Polyclonal Antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Corylin improves the TLR4 signal pathway in DSS-induced colitis mice. ( A ) Expression analysis by immunohistochemical staining of TLR4, p-p38, and AP-1 in mice after the indicated treatment. Scale bar: 100 mum. Red arrows highlight positive staining. ( B ) Western blot analysis of TLR4, MyD88, p-p38, AP-1, and beta-actin (loading control) in colon homogenates. Right graph indicates quantification relative to beta-actin. ( C , D ) mRNA expression of Ifngamma , Tnf-alpha , Il-6 , Il-1beta , Nlrp3 , Asc , Pannexin , and Pro-caspase 1 determined by qRT-PCR. ( E , F ) Western blot analysis of IFNgamma, TNF-alpha, IL-6, IL-1beta, NLRP3, ASC, Pannexin, and Caspase 1, and beta-actin (loading control) in colon homogenates. Results represent mean +- SEM. * p < 0.05, Normal compared with DSS; # p < 0.05, DSS compared with DSS + Corylin (L); Y= p < 0.05, DSS compared with DSS + Corylin (H); SS p < 0.05, DSS + Corylin (L) compared with DSS + Corylin (H). DSS, dextran sodium sulfate; TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response 88; AP-1, activator protein 1; Ifngamma , interferon gamma; Tnf-alpha , tumor necrosis factor-alpha; Il-6 , interleukin-6; Nlrp3 , NACHT, LRR, and PYD domains-containing protein 3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Corylin improves the TLR4 signaling pathway in AOM/DSS-induced-colitis-associated colorectal cancer mice. ( A ) Expression analysis by immunohistochemical staining of TLR4, p-p38, and AP-1 in mice after the indicated treatment. Scale bar: 100 mum. ( B ) Western blot analysis of TLR4, MyD88, p-p38, AP-1, and beta-actin (loading control) in colon. ( C , D ) mRNA expression of Ifngamma , Tnf-alpha , Il-6 , Il-1beta , Nlrp3 , Asc , Pannexin , and Pro-caspase 1 as determined by qRT-PCR. ( E , F ) Western blot analysis of IFNgamma, TNF-alpha, IL-6, IL-1beta, NLRP3, ASC, Pannexin, and Caspase 1, and beta-actin (loading control) in colon homogenates. Results represent mean +- SEM. * p < 0.05, Normal compared with AOM/DSS; # p < 0.05, AOM/DSS compared with AOM/DSS + Corylin (L); Y= p < 0.05, AOM/DSS compared with AOM/DSS + Corylin (H); SS p < 0.05, DSS + Corylin (L) compared with DSS + Corylin (H). AOM, azoxymethane; DSS, dextran sodium sulfate; TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response 88; AP-1, activator protein 1; Ifngamma , interferon gamma; Tnf-alpha , tumor necrosis factor-alpha; Il-6 , interleukin-6; Nlrp3 , NACHT, LRR, and PYD domains-containing protein 3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Treatment with IP-Se-06 (1 mu M) at 48 h downregulated p38 mitogen-activated protein kinase and inflammasome complex protein in A172 glioblastoma cells. (a) Immunoblotting analysis detected the downregulation of p38, p-p38, NLRP3 and caspase-1 protein levels. beta -Actin was used as a loading control. (b) Densitometric analysis results of p38, p-p38, NLRP3 and caspase-1 were quantified with ImageJ. The data were considered as statistically significant at * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the control group (nontreated cells).

PA5-87536

Antibody data