MAB3427

antibody from Abnova Corporation

Targeting: CASP1 ICE, IL1BC

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3427

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB3427, RRID:AB_10632177

Product name

Casp1 monoclonal antibody, clone 1H11

Antibody type

Monoclonal

Description

Rat monoclonal antibody raised against synthetic peptide of Casp1.

Antigen sequence

TALEMVKEVKEFAAC


Isotype

IgG

Antibody clone number

1H11

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse Casp1 as a band of ~45 kDa.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect endogenous pro-Casp1 in spleen from WT mice, but not from spleen of Casp1 deficient mice. HSP-70 : loading control.

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Casp1 monoclonal antibody, clone 1H11 (Cat # MAB3427) detect overexpressed Casp1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse Casp1 by flow cytometry.

Validation comment

Flow Cytometry