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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA1-41214 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: antigen standard for CASP1 (transient overexpression lysate), Hela whole cell extract.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-1 on Human HeLa lysate using a Caspase 1 polyclonal antibody (Product # PA1-41214) at 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout validation by Western blot analysis of Caspase 1 in Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. Samples were incubated in Caspase 1 polyclonal antibody (Product # PA1-41214) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 1 in Human HeLa lysate. Samples were incubated in Caspase 1 polyclonal antibody (Product # PA1-41214) using a dilution of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Caspase 1 in Human reactive lymph node. Samples were incubated in Caspase 1 polyclonal antibody (Product # PA1-41214) followed by a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.
