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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-20113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Hela cell lysate.
- Concentration
- 1 mg/mL
Submitted references Myocardial hypothermia increases autophagic flux, mitochondrial mass and myocardial function after ischemia-reperfusion injury.
Marek-Iannucci S, Thomas A, Hou J, Crupi A, Sin J, Taylor DJ, Czer LS, Esmailian F, Mentzer RM Jr, Andres AM, Gottlieb RA
Scientific reports 2019 Jul 10;9(1):10001
Scientific reports 2019 Jul 10;9(1):10001
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa cell lysate using a Caspase-1 polyclonal antibody (Product # PA5-20113) at (A) 0.5, (B) 1, and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Caspase-1 in HeLa cell lysate with Caspase 1 Polyclonal Antibody (Product # PA5-20113) (IN) at (A) 0.5, (B) 1, and (C) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a Caspase-1 polyclonal antibody (Product # PA5-20113) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Caspase-1 in Hela cells with Caspase 1 Polyclonal Antibody (Product # PA5-20113) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of caspase-1 in human spleen tissue with Caspase 1 Polyclonal Antibody (Product # PA5-20113) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 Myocardial hypothermia decreases local and systemic inflammation. Myocardial border zone tissue of female farm pigs (n = 3 per group) was harvested one week post MI. ( A , B ) Quantification of TNFalpha and TGFbeta mRNA expression respectively. ( C ) Quantification of local tissue inflammation via iNOS and Arginase-1 staining (representative images: Supplemental Fig. 2 ). ( D ) WB analysis with protein quantification ( E - G ) of iNOS, Arginase-1 and Caspase-1 respectively in whole lysate and ( H ) pNFkappaB in the nuclear fraction. ( I ) Quantification of lymphocyte proliferation with Concanavalin-A treatment on fresh splenic tissue, harvested from the same pigs. The data represents the mean +- SEM with *p < 0.05, **p < 0.01, comparing cold (black) vs. warm (white). We normalized each protein to the entire corresponding lane on the ponceau-stained membrane, representing the total protein amount. Due to space limitation, the blots are cropped from various gels, please see the supplemental data for full-length gels/blots.
