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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27387 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116, THP-1 conditioned medium (500 ng/mL LPS treatment for 4 hr and 1 mM ATP treatment for 1.5hr). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 1 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Caspase 1 polyclonal antibody (Product # PA5-27387) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Caspase 1 was performed by separating treated (+) THP-1 conditioned medium (30 µg) by 15% SDS-PAGE. The membrane was blotted with Caspase 1 Polyclonal Antibody (Product # PA5-27387) diluted at 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Caspase 1 Polyclonal Antibody (Product # PA5-27387). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 1 Polyclonal Antibody (Product # PA5-27387) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Caspase 1 in methanol-fixed HeLa cells using a Caspase 1 polyclonal antibody (Product # PA5-27387) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Caspase 1 Polyclonal Antibody detects Caspase 1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Caspase 1 stained by Caspase 1 Polyclonal Antibody (Product # PA5-27387) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha (Product # PA5-27387) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
