PA5-29342

antibody from Invitrogen Antibodies

Targeting: CASP1 ICE, IL1BC

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29342

Provider

Invitrogen Antibodies

Product name

Caspase 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Diacerein counteracts acetaminophen-induced hepatotoxicity in mice via targeting NLRP3/caspase-1/IL-1β and IL-4/MCP-1 signaling pathways.

Elshal M, Abdelmageed ME
Archives of pharmacal research 2022 Mar;45(3):142-158

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A promiscuous inflammasome sparks replication of a common tumor virus.

Burton EM, Goldbach-Mansky R, Bhaduri-McIntosh S
Proceedings of the National Academy of Sciences of the United States of America 2020 Jan 21;117(3):1722-1730

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Caspase 1 in methanol-fixed HeLa cells using a Caspase 1 polyclonal antibody (Product # PA5-29342) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Caspase 1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Caspase 1 Polyclonal Antibody (Product # PA5-29342) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Caspase 1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Caspase 1 Polyclonal Antibody (Product # PA5-29342) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Caspase 1 (Product # PA5-29342) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Effect of diacerein (DCN) on A NLR family pyrin domain containing 3 (NLRP3) and B Interlukin-1 beta (IL-1beta) levels in hepatic tissue of acetaminophen-injected mice. Data are expressed as mean +- SEM (n = 8). #p < 0.05, ##p < 0.01 compared to control group, *p < 0.05, **p < 0.01 compared to acetaminophen-injected group, Fp < 0.05, FFp < 0.01 compared to DCN 4 h prior to acetaminophen group, *p < 0.05, **p < 0.01 compared to DCN 2 h post-acetaminophen group using one-way ANOVA followed by Tukey-Kramer multiple comparisons post hoc test. C Effect of diacerein (DCN) on hepatic caspase-1 assessed by immunohistochemistry (X 400, bar = 50 um); (a) hepatic sections of control group showing negative staining; (b) hepatic sections of DCN group showing negative staining; (c) hepatic sections of acetaminophen group showing marked positive brown cytoplasmic expression in hepatocytes (black arrows) associated with area of lesions around central veins; (d) hepatic sections of DCN 4 h prior to acetaminophen group showing a significant reduction of caspase-1 cytoplasmic expression (black arrows); (e) hepatic sections of DCN 2 h post-acetaminophen group showing a significant reduction of caspase-1 cytoplasmic expression (black arrows), and (f) scatter dot plots of the immunohistopathological assessment of hepatic caspase-1 scores. $p < 0.05, $$p < 0.01 compared to control group, +p < 0.05, ++p < 0.01 compared to compared to acetaminophen group using Kruskal-Wallis followed by Dunn's Multipl