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Western blot
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-531-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-531-C100, RRID:AB_2050922
- Product name
- Caspase-1 (mouse) monoclonal antibody (4G8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 4G8
- Vial size
- 100 μg
- Storage
- -20°C
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Figure A: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse caspase-1 by flow cytometry. Figure B: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse caspase-1 as a band of ~45 kDa. Figure C: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect endogenous pro-caspase-1 in spleen from WT mice, but not from spleen of caspase-1 deficient mice. HSP-70: loading control.
