NB100-2225

antibody from Novus Biologicals

Targeting: DDX24

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2225

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2225, RRID:AB_2246001

Product name

Rabbit Polyclonal DDX24 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX24 Antibody [NB100-2225] - Detection of Human DDX24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T and NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX24 antibody NB100-2225 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DDX24 Antibody [NB100-2225] - Detection of Human and Mouse DDX24 on HeLa whole cell lystate using NB100-2225. DDX24 was also immunoprecipitated by rabbit anti-DDX24 antibody NB100-2224 and NB100-2225.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: DDX24 Antibody [NB100-2225] - Detection of human DDX24 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX24 antibody NB100-2225 (lot 2) used for IP at 6 ug per reaction. DDX24 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated DDX24, NB100-2225 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.