Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Protein array [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- V3884 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Spectrin alpha 1 Antibody / SPTA1
- Antibody type
- Monoclonal
- Description
- This highly specific Spectrin alpha 1 antibody is suitable for use in ELISA/Western blot. Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- SPTA1/1832
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the Spectrin alpha 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil with Spectrin alpha 1 antibody (clone SPTA1/1832). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placenta with Spectrin alpha 1 antibody (clone SPTA1/1832). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Spectrin alpha 1 antibody (clone SPTA1/1832). These results demonstrate the foremost specificity of the SPTA1/1832 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Spectrin alpha 1 antibody (clone SPTA1/1832) as confirmation of integrity and purity.
