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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB100-2070 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2070, RRID:AB_10000708
- Product name
- Mouse Monoclonal Spectrin alpha 1 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. Localizes the 220 kDa (beta) and 240 kDa (alpha) bands of human erythrocyte spectrin staining the beta subunit stronger than the alpha subunit at the recommended titer. In addition, a number of polypeptides in the region of 170, 150, 85, and 45 kDa will react with the antibody. It is likely these lower molecular weight polypeptides may have an epitope shared by both the alpha and beta subunits of spectrin. By immunofluorescence the antibody will stain human spectrin in erythrocyte smears.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Transforming Growth Factor-β1 Antagonizes Interleukin-5 Pro-Survival Signaling by Activating Calpain-1 in Primary Human Eosinophils.
Xie Q, Shen ZJ, Oh J, Chu H, Malter JS
Journal of clinical & cellular immunology 2011 Nov 15;Suppl 1
Journal of clinical & cellular immunology 2011 Nov 15;Suppl 1
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Spectrin alpha 1 Antibody (SB-SP1) [NB100-2070] - Erythrocytes ghost extract was separated on SDS-PAGE and probed with Monoclonal AB To Spectrin (MO) CL:SB-SP1 (Prepack). The antibody was developed with HRP APA Mouse Fab ads/HIgG and AEC substrate. Antibody dilution 1/1,000.
