- PA5-28266 - Invitrogen Antibodies PRKAR2B antibody

Submitted references Melanoma-Secreted Amyloid Beta Suppresses Neuroinflammation and Promotes Brain Metastasis.

Kleffman K, Levinson G, Rose IVL, Blumenberg LM, Shadaloey SAA, Dhabaria A, Wong E, Galán-Echevarría F, Karz A, Argibay D, Von Itter R, Floristán A, Baptiste G, Eskow NM, Tranos JA, Chen J, Vega Y Saenz de Miera EC, Call M, Rogers R, Jour G, Wadghiri YZ, Osman I, Li YM, Mathews P, DeMattos RB, Ueberheide B, Ruggles KV, Liddelow SA, Schneider RJ, Hernando E
Cancer discovery 2022 May 2;12(5):1314-1335

PRKAR2B-HIF-1α loop promotes aerobic glycolysis and tumour growth in prostate cancer.

Xia L, Sun J, Xie S, Chi C, Zhu Y, Pan J, Dong B, Huang Y, Xia W, Sha J, Xue W
Cell proliferation 2020 Nov;53(11):e12918

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of PKA 2 beta in methanol-fixed HeLa cells using a PKA 2 beta polyclonal antibody (Product # PA5-28266) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 4 PRKAR2B regulates HIF1alpha expression in prostate cancer. (A-B) Correlation analysis of the link between PRKAR2B and HIF1A expression in prostate cancer tissues; data were obtained from the TCGA cohort (A) and Ren Ji cohort (B). (C) Western blotting analysis of the effect of PRKAR2B knockdown on the HIF1A protein level in DU145 cells. (D) Western blotting analysis of the effect of PRKAR2B overexpression on the HIF1A protein level in LNCaP cells. (E) Western blotting analysis of HIF1A protein level in LNCaP and LNCaP-AI cells. (F) Western blotting analysis of the effect of PRKAR2B knockdown on the HIF1A protein level in LNCaP-AI cells. (G) Effects of PRKAR2B knockdown or overexpression on the HIF-1alpha activity. (H) Western blotting analysis of HIF1A protein level in DU145 and PC3 cells upon H89 (50 mumol/L) treatment for 24 h. (I) Determining the effect of H89 (50 mumol/L) treatment on the HIF-1alpha activity in DU145 and PC3 cells. * P < .05; ** P < .01

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 1 FIGURE PRKAR2B knockdown inhibits prostate cancer cell glycolysis. A, Western blotting analysis of the knockdown efficiency of PRKAR2B in DU145 and PC3 cells. B-E, The effect of PRKAR2B knockdown on the glucose utilization (B), lactate production (C), extracellular acidification rate (D), oxygen consumption rate (E), and expression of glycolytic components (F) in DU145 and PC3 cells. * P < .05; ** P < .01

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 2 FIGURE PRKAR2B overexpression promotes the Warburg effect in prostate cancer in vitro. A, Western blotting analysis of the overexpression efficiency of PRKAR2B in LNCaP cells. B-E, The effect of PRKAR2B overexpression on the glucose utilization (B), lactate production (C), extracellular acidification rate (D), oxygen consumption rate (E), and expression of glycolytic components (F) in LNCaP cells. SLC2A1, solute carrier family 2 member 1; PFKP, phosphofructokinase platelet; PKM, pyruvate kinase muscle isozyme; LDHA, lactate dehydrogenase A. * P < .05; ** P < .01

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 FIGURE Androgen-independent LNCaP cells exhibits enhanced glycolysis. A, The basal glucose utilization in LNCaP and LNCaP-AI cells. B, The basal lactate production in LNCaP and LNCaP-AI cells. C, Comparison of ECAR and OCR status in LNCaP and LNCaP-AI cells. D, Real-time qPCR analysis of glucose transporter and key glycolytic genes in LNCaP and LNCaP-AI cells. E, Western blotting analysis of the effect of PRKAR2B knockdown efficiency in LNCaP-AI cells. (F-I) The effect of PRKAR2B knockdown on the glucose utilization (F), lactate production (G), ECAR/OCR (H), and expression of glycolytic components (I) in LNCaP-AI cells. (J) Correlation analysis of the link between PRKAR2B expression and the expression level of glucose transporter and key glycolytic genes in prostate cancer tissues (n = 492); data were obtained from the TCGA cohort. * P < .05; ** P < .01

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 4 FIGURE PRKAR2B regulates HIF1alpha expression in prostate cancer. (A-B) Correlation analysis of the link between PRKAR2B and HIF1A expression in prostate cancer tissues; data were obtained from the TCGA cohort (A) and Ren Ji cohort (B). (C) Western blotting analysis of the effect of PRKAR2B knockdown on the HIF1A protein level in DU145 cells. (D) Western blotting analysis of the effect of PRKAR2B overexpression on the HIF1A protein level in LNCaP cells. (E) Western blotting analysis of HIF1A protein level in LNCaP and LNCaP-AI cells. (F) Western blotting analysis of the effect of PRKAR2B knockdown on the HIF1A protein level in LNCaP-AI cells. (G) Effects of PRKAR2B knockdown or overexpression on the HIF-1alpha activity. (H) Western blotting analysis of HIF1A protein level in DU145 and PC3 cells upon H89 (50 mumol/L) treatment for 24 h. (I) Determining the effect of H89 (50 mumol/L) treatment on the HIF-1alpha activity in DU145 and PC3 cells. * P < .05; ** P < .01

PA5-28266

Antibody data