PA5-27263

antibody from Invitrogen Antibodies

Targeting: RRM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27263

Provider

Invitrogen Antibodies

Product name

RRM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (87%), Xenopus laevis (86%), Chicken (92%), Rhesus Monkey (98%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RRM1 using 30 µg of 293T lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a RRM1 polyclonal antibody (Product # PA5-27263) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RRM1 Polyclonal Antibody (Product # PA5-27263). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 Polyclonal Antibody (Product # PA5-27263) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RRM1 Polyclonal Antibody detects RRM1 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract. B. 30 µg Raw264.7 whole cell lysate/extract. C. 30 µg C2C12 whole cell lysate/extract.5 % SDS-PAGE. RRM1 Polyclonal Antibody (Product # PA5-27263) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RRM1 Polyclonal Antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RRM1 stained by RRM1 Polyclonal Antibody (Product # PA5-27263) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RRM1 Polyclonal Antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: RRM1 protein stained by RRM1 Polyclonal Antibody (Product # PA5-27263) diluted at 1:1,000. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RRM1 Polyclonal Antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RRM1 stained by RRM1 Polyclonal Antibody (Product # PA5-27263) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RRM1 (Product # PA5-27263) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RRM1 Polyclonal Antibody detects RRM1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. RRM1 stained by RRM1 Polyclonal Antibody (Product # PA5-27263) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RRM1 Polyclonal Antibody detects RRM1 protein on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. RRM1 Polyclonal Antibody (Product # PA5-27263) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.