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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP8145b - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP8145b, RRID:AB_2283722
- Product name
- PFKFB3 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Insulin-independent promotion of chemically induced hepatocellular tumor development in genetically diabetic mice.
Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3.
Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth.
Ras transformation requires metabolic control by 6-phosphofructo-2-kinase.
Yamasaki K, Hayashi Y, Okamoto S, Osanai M, Lee GH
Cancer science 2010 Jan;101(1):65-72
Cancer science 2010 Jan;101(1):65-72
Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3.
Ando M, Uehara I, Kogure K, Asano Y, Nakajima W, Abe Y, Kawauchi K, Tanaka N
Journal of Nippon Medical School = Nippon Ika Daigaku zasshi 2010 Apr;77(2):97-105
Journal of Nippon Medical School = Nippon Ika Daigaku zasshi 2010 Apr;77(2):97-105
Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth.
Clem B, Telang S, Clem A, Yalcin A, Meier J, Simmons A, Rasku MA, Arumugam S, Dean WL, Eaton J, Lane A, Trent JO, Chesney J
Molecular cancer therapeutics 2008 Jan;7(1):110-20
Molecular cancer therapeutics 2008 Jan;7(1):110-20
Ras transformation requires metabolic control by 6-phosphofructo-2-kinase.
Telang S, Yalcin A, Clem AL, Bucala R, Lane AN, Eaton JW, Chesney J
Oncogene 2006 Nov 23;25(55):7225-34
Oncogene 2006 Nov 23;25(55):7225-34
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Western blot analysis of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469) (Cat. #AP8145b). AP8145b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PFKFB3 Antibody (C-term)(Cat. #AP8145b) in CEM cell line lysates (35ug/lane). PFKFB3(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
