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- Antibody Data
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- References [12]
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- Western blot [2]
- ELISA [1]
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- Product number
- H00005209-M08 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005209-M08, RRID:AB_1146566
- Product name
- Mouse Monoclonal 6 Phosphofructo 2 Kinase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PFKFB3 - 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (3F3)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A novel human Cdh1 mutation impairs anaphase promoting complex/cyclosome activity resulting in microcephaly, psychomotor retardation, and epilepsy.
Targeting PFKFB3 alleviates cerebral ischemia-reperfusion injury in mice.
Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.
MAPK14/p38α-dependent modulation of glucose metabolism affects ROS levels and autophagy during starvation.
Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the pentose phosphate pathway.
Energy management by enhanced glycolysis in G1-phase in human colon cancer cells in vitro and in vivo.
Increased concentrations of fructose 2,6-bisphosphate contribute to the Warburg effect in phosphatase and tensin homolog (PTEN)-deficient cells.
Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration.
Phospho-ΔNp63α/SREBF1 protein interactions: bridging cell metabolism and cisplatin chemoresistance.
E3 ubiquitin ligase APC/C-Cdh1 accounts for the Warburg effect by linking glycolysis to cell proliferation.
Role of oxysterol binding protein in hepatitis C virus infection.
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Rodríguez C, Sánchez-Morán I, Álvarez S, Tirado P, Fernández-Mayoralas DM, Calleja-Pérez B, Almeida Á, Fernández-Jaén A
Journal of neurochemistry 2019 Oct;151(1):103-115
Journal of neurochemistry 2019 Oct;151(1):103-115
Targeting PFKFB3 alleviates cerebral ischemia-reperfusion injury in mice.
Burmistrova O, Olias-Arjona A, Lapresa R, Jimenez-Blasco D, Eremeeva T, Shishov D, Romanov S, Zakurdaeva K, Almeida A, Fedichev PO, Bolaños JP
Scientific reports 2019 Aug 12;9(1):11670
Scientific reports 2019 Aug 12;9(1):11670
Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.
Ohmura M, Hishiki T, Yamamoto T, Nakanishi T, Kubo A, Tsuchihashi K, Tamada M, Toue S, Kabe Y, Saya H, Suematsu M
Nitric oxide : biology and chemistry 2015 Apr 30;46:102-13
Nitric oxide : biology and chemistry 2015 Apr 30;46:102-13
MAPK14/p38α-dependent modulation of glucose metabolism affects ROS levels and autophagy during starvation.
Desideri E, Vegliante R, Cardaci S, Nepravishta R, Paci M, Ciriolo MR
Autophagy 2014 Sep;10(9):1652-65
Autophagy 2014 Sep;10(9):1652-65
Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the pentose phosphate pathway.
Yamamoto T, Takano N, Ishiwata K, Ohmura M, Nagahata Y, Matsuura T, Kamata A, Sakamoto K, Nakanishi T, Kubo A, Hishiki T, Suematsu M
Nature communications 2014 Mar 17;5:3480
Nature communications 2014 Mar 17;5:3480
Energy management by enhanced glycolysis in G1-phase in human colon cancer cells in vitro and in vivo.
Bao Y, Mukai K, Hishiki T, Kubo A, Ohmura M, Sugiura Y, Matsuura T, Nagahata Y, Hayakawa N, Yamamoto T, Fukuda R, Saya H, Suematsu M, Minamishima YA
Molecular cancer research : MCR 2013 Sep;11(9):973-85
Molecular cancer research : MCR 2013 Sep;11(9):973-85
Increased concentrations of fructose 2,6-bisphosphate contribute to the Warburg effect in phosphatase and tensin homolog (PTEN)-deficient cells.
Cordero-Espinoza L, Hagen T
The Journal of biological chemistry 2013 Dec 13;288(50):36020-8
The Journal of biological chemistry 2013 Dec 13;288(50):36020-8
Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration.
Rodriguez-Rodriguez P, Fernandez E, Almeida A, Bolaños JP
Cell death and differentiation 2012 Oct;19(10):1582-9
Cell death and differentiation 2012 Oct;19(10):1582-9
Phospho-ΔNp63α/SREBF1 protein interactions: bridging cell metabolism and cisplatin chemoresistance.
Huang Y, Bell LN, Okamura J, Kim MS, Mohney RP, Guerrero-Preston R, Ratovitski EA
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
E3 ubiquitin ligase APC/C-Cdh1 accounts for the Warburg effect by linking glycolysis to cell proliferation.
Almeida A, Bolaños JP, Moncada S
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 12;107(2):738-41
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 12;107(2):738-41
Role of oxysterol binding protein in hepatitis C virus infection.
Amako Y, Sarkeshik A, Hotta H, Yates J 3rd, Siddiqui A
Journal of virology 2009 Sep;83(18):9237-46
Journal of virology 2009 Sep;83(18):9237-46
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Analysis of PFKFB3 expression in transfected 293T cell line by PFKFB3 monoclonal antibody (M08), clone 3F3. Lane 1: PFKFB3 transfected lysatE (59.6 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Analysis of PFKFB3 transfected lysate using anti-PFKFB3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PFKFB3 rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: 6 Phosphofructo 2 Kinase Antibody (3F3) [H00005209-M08] - Detection limit for recombinant GST tagged PFKFB3 is approximately 3ng/ml as a capture antibody.
