Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-96444 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRPS30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PHNPALAAAA LDYRCPVHFY WVRGEEIIPR GHRRGRIDDL RYQIDDKPNN QIRISKQLAE FVPLDYSVPI EIPTIKCKPD KLPLFKRQYE NHIFVGSKTA DPCCYGHTQF HLLPDKLRRE RLLRQNCADQ IEVVFRANAI ASLFAWTGAQ AMYQGFWSEA DVTRPFVSQA VITDGKYFSF FCYQLNTLAL TTQADQNNPR KNICWGTQSK PLYETIEDND VKGFNDDVLL QIVHFLLNRP KEEKSQLLEN; Positive Samples: SW620, HL-60, HeLa, HepG2; Cellular Location: Mitochondrion
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.89 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRPS30 in extracts of various cell lines. Samples were incubated with MRPS30 Polyclonal antibody (Product # PA5-96444) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MRPS30 was performed in U2OS cells using MRPS30 Polyclonal Antibody (Product # PA5-96444).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MRPS30 in U2OS cells. Samples were incubated with MRPS30 Polyclonal antibody (Product # PA5-96444). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MRPS30 in 200 μg extracts of HepG2 cells. Samples were precipitated with 1 μg MRPS30 Polyclonal antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal antibody (Product # PA5-96444) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of MRPS30 was performed in 200 µg extracts of HepG2 cells using MRPS30 Polyclonal Antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal Antibody at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of MRPS30 was performed in 200 µg extracts of HepG2 cells using MRPS30 Polyclonal Antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal Antibody at a dilution of 1:1000.