PA5-96444

antibody from Invitrogen Antibodies

Targeting: MRPS30 PAP, PDCD9

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96444

Provider

Invitrogen Antibodies

Product name

MRPS30 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PHNPALAAAA LDYRCPVHFY WVRGEEIIPR GHRRGRIDDL RYQIDDKPNN QIRISKQLAE FVPLDYSVPI EIPTIKCKPD KLPLFKRQYE NHIFVGSKTA DPCCYGHTQF HLLPDKLRRE RLLRQNCADQ IEVVFRANAI ASLFAWTGAQ AMYQGFWSEA DVTRPFVSQA VITDGKYFSF FCYQLNTLAL TTQADQNNPR KNICWGTQSK PLYETIEDND VKGFNDDVLL QIVHFLLNRP KEEKSQLLEN; Positive Samples: SW620, HL-60, HeLa, HepG2; Cellular Location: Mitochondrion

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.89 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRPS30 in extracts of various cell lines. Samples were incubated with MRPS30 Polyclonal antibody (Product # PA5-96444) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MRPS30 was performed in U2OS cells using MRPS30 Polyclonal Antibody (Product # PA5-96444).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MRPS30 in U2OS cells. Samples were incubated with MRPS30 Polyclonal antibody (Product # PA5-96444). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of MRPS30 in 200 μg extracts of HepG2 cells. Samples were precipitated with 1 μg MRPS30 Polyclonal antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal antibody (Product # PA5-96444) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of MRPS30 was performed in 200 µg extracts of HepG2 cells using MRPS30 Polyclonal Antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal Antibody at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of MRPS30 was performed in 200 µg extracts of HepG2 cells using MRPS30 Polyclonal Antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal Antibody at a dilution of 1:1000.