PA5-96444

antibody from Invitrogen Antibodies

Targeting: MRPS30 PAP, PDCD9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96444

Provider

Invitrogen Antibodies

Product name

MRPS30 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PHNPALAAAA LDYRCPVHFY WVRGEEIIPR GHRRGRIDDL RYQIDDKPNN QIRISKQLAE FVPLDYSVPI EIPTIKCKPD KLPLFKRQYE NHIFVGSKTA DPCCYGHTQF HLLPDKLRRE RLLRQNCADQ IEVVFRANAI ASLFAWTGAQ AMYQGFWSEA DVTRPFVSQA VITDGKYFSF FCYQLNTLAL TTQADQNNPR KNICWGTQSK PLYETIEDND VKGFNDDVLL QIVHFLLNRP KEEKSQLLEN; Positive Samples: SW620, HL-60, HeLa, HepG2; Cellular Location: Mitochondrion

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.89 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MRPS30 in extracts of various cell lines using MRPS30 Polyclonal Antibody (Product # PA5-96444) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MRPS30 was performed in U2OS cells using MRPS30 Polyclonal Antibody (Product # PA5-96444).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MRPS30 in paraffin-embedded rat lung using MRPS30 Polyclonal Antibody (Product # PA5-96444) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MRPS30 in paraffin-embedded mouse kidney using MRPS30 Polyclonal Antibody (Product # PA5-96444) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of MRPS30 was performed in 200 µg extracts of HepG2 cells using MRPS30 Polyclonal Antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal Antibody at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of MRPS30 was performed in 200 µg extracts of HepG2 cells using MRPS30 Polyclonal Antibody (Product # PA5-96444). Western blot was performed from the immunoprecipitate using MRPS30 Polyclonal Antibody at a dilution of 1:1000.