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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA010703 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA010703, RRID:AB_10600806
- Product name
- Anti-DNASE1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human DNASE1, Gene description: deoxyribonuclease I, Alternative Gene Names: DNL1, Validated applications: WB, IHC, Uniprot ID: P24855, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Ocular surface extracellular DNA and nuclease activity imbalance: a new paradigm for inflammation in dry eye disease.
Sonawane S, Khanolkar V, Namavari A, Chaudhary S, Gandhi S, Tibrewal S, Jassim SH, Shaheen B, Hallak J, Horner JH, Newcomb M, Sarkar J, Jain S
Investigative ophthalmology & visual science 2012 Dec 17;53(13):8253-63
Investigative ophthalmology & visual science 2012 Dec 17;53(13):8253-63
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and DNASE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401601).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human duodenum and liver tissues using HPA010703 antibody. Corresponding DNASE1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
