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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX109306 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109306, RRID:AB_1950133
- Product name
- DNase I antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Taming hyperactive hDNase I: Stable inducible expression of a hyperactive salt- and actin-resistant variant of human deoxyribonuclease I in CHO cells.
Lam C, Santell L, Wilson B, Yim M, Louie S, Tang D, Shaw D, Chan P, Lazarus RA, Snedecor B, Misaghi S
Biotechnology progress 2017 Mar;33(2):523-533
Biotechnology progress 2017 Mar;33(2):523-533
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: MOLT4 (GTX27912)10% SDS PAGEGTX109306 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with DNase I antibody [N1C3] (GTX109306) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: DNase I protein stained by DNase I antibody [N1C3] (GTX109306) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. DNase I antibody [N1C3] (GTX109306) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. DNase I antibody [N1C3] (GTX109306) diluted at 1:500.
