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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R31960 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Integrin alpha 4 Antibody / ITGA4
- Antibody type
- Polyclonal
- Description
- This highly specific Integrin alpha 4 antibody is suitable for use in Western blot/Immunohistochemistry applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Integrin alpha 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat kidney, human 3) Jurkat, 4) 22RV1, 5) HepG2, 6) SMMC lysate with Integrin alpha 4 antibody. Predicted molecular weight ~115 kDa, routinely observed at ~150 kDa; observed here at ~220 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer with Integrin alpha 4 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
