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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Flow cytometry [3]
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- Product number
- MA49D7 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITGA4 Monoclonal Antibody (TA-2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA49D7 targets CD49d in FACS, IHC, and Neu applications and shows reactivity with Rat and Human samples.
- Antibody clone number
- TA-2
- Concentration
- 1.0 mg/mL
Submitted references Resting CD4+ effector memory T cells are precursors of bystander-activated effectors: a surrogate model of rheumatoid arthritis synovial T-cell function.
Selectins and integrins but not platelet-endothelial cell adhesion molecule-1 regulate opioid inhibition of inflammatory pain.
VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.
VCAM-1 contributes to rapid eosinophil accumulation induced by the chemoattractants PAF and LTB4: evidence for basal expression of functional VCAM-1 in rat skin.
VCAM-1 contributes to rapid eosinophil accumulation induced by the chemoattractants PAF and LTB4: evidence for basal expression of functional VCAM-1 in rat skin.
Lymphocyte subpopulations in lymphoid organs of rats after acute resistance exercise.
Effect of a new monoclonal antibody, TA-2, that inhibits lymphocyte adherence to cytokine stimulated endothelium in the rat.
Inhibition of in vivo lymphocyte migration to inflammation and homing to lymphoid tissues by the TA-2 monoclonal antibody. A likely role for VLA-4 in vivo.
Brennan FM, Smith NM, Owen S, Li C, Amjadi P, Green P, Andersson A, Palfreeman AC, Hillyer P, Foey A, Beech JT, Feldmann M
Arthritis research & therapy 2008;10(2):R36
Arthritis research & therapy 2008;10(2):R36
Selectins and integrins but not platelet-endothelial cell adhesion molecule-1 regulate opioid inhibition of inflammatory pain.
Machelska H, Brack A, Mousa SA, Schopohl JK, Rittner HL, Schäfer M, Stein C
British journal of pharmacology 2004 Jun;142(4):772-80
British journal of pharmacology 2004 Jun;142(4):772-80
VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.
Larbi KY, Allen AR, Tam FW, Haskard DO, Lobb RR, Silva PM, Nourshargh S
Blood 2000 Nov 15;96(10):3601-9
Blood 2000 Nov 15;96(10):3601-9
VCAM-1 contributes to rapid eosinophil accumulation induced by the chemoattractants PAF and LTB4: evidence for basal expression of functional VCAM-1 in rat skin.
Davies D, Larbi K, Allen A, Sanz M, Weg VB, Haskard DO, Lobb RR, Nourshargh S
Immunology 1999 May;97(1):150-8
Immunology 1999 May;97(1):150-8
VCAM-1 contributes to rapid eosinophil accumulation induced by the chemoattractants PAF and LTB4: evidence for basal expression of functional VCAM-1 in rat skin.
Davies D, Larbi K, Allen A, Sanz M, Weg VB, Haskard DO, Lobb RR, Nourshargh S
Immunology 1999 May;97(1):150-8
Immunology 1999 May;97(1):150-8
Lymphocyte subpopulations in lymphoid organs of rats after acute resistance exercise.
Mastro AM, Schlosser DA, Grove DS, Lincoski C, Pishak SA, Gordon S, Kraemer WJ
Medicine and science in sports and exercise 1999 Jan;31(1):74-81
Medicine and science in sports and exercise 1999 Jan;31(1):74-81
Effect of a new monoclonal antibody, TA-2, that inhibits lymphocyte adherence to cytokine stimulated endothelium in the rat.
Issekutz TB, Wykretowicz A
Journal of immunology (Baltimore, Md. : 1950) 1991 Jul 1;147(1):109-16
Journal of immunology (Baltimore, Md. : 1950) 1991 Jul 1;147(1):109-16
Inhibition of in vivo lymphocyte migration to inflammation and homing to lymphoid tissues by the TA-2 monoclonal antibody. A likely role for VLA-4 in vivo.
Issekutz TB
Journal of immunology (Baltimore, Md. : 1950) 1991 Dec 15;147(12):4178-84
Journal of immunology (Baltimore, Md. : 1950) 1991 Dec 15;147(12):4178-84
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD49d in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD49d in THP-1 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD49d in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
