MA49D7

antibody from Invitrogen Antibodies

Targeting: ITGA4 CD49D

Immunohistochemistry

Blocking/Neutralizing

Antibody data

Submit

Validation data

Product number: MA49D7 - Provider product page
Provider: Invitrogen Antibodies
Product name: ITGA4 Monoclonal Antibody (TA-2)
Antibody type: Monoclonal
Antigen: Other
Description: MA49D7 targets CD49d in FACS, IHC, and Neu applications and shows reactivity with Rat and Human samples. The MA49D7 immunogen is rat 150 kD alpha4 chain of VLA-4 (CD49d). MA49D7 detects CD49d which has a predicted molecular weight of approximately 111 kDa. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
Reactivity: Human, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: TA-2
Vial size: 500 µg
Concentration: 1.0 mg/mL
Storage: -20°C

ITGA4 protein structure - MA49D7 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD49d in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD49d in THP-1 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD49d in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.