R32307

antibody from NSJ Bioreagents

Targeting: ITCH AIP4

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32307

Provider

NSJ Bioreagents

Product name

ITCH Antibody / AIF4

Antibody type

Polyclonal

Description

This highly specific ITCH antibody is suitable for use in Western blot/Flow cytometry applications with human, mouse and rat, monkey samples.

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the ITCH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) Raji and 3) U937 lysate with ITCH antibody. Predicted molecular weight: ~99 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human K562, 2) human Raji, 3) human 293T, 4) monkey COS7, 5) human HepG2, 6) human A549, 7) rat C6 and 8) mouse NIH 3T3 cell lysate with ITCH antibody. Predicted molecular weight: 86/99/102 kDa (multiple isoforms).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human SiHa cells with ITCH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ITCH antibody.