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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PAB7215 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7215, RRID:AB_1576419
- Product name
- ITCH polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of ITCH.
- Antigen sequence
C-EIKSHDLKPNGGN- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination.
Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A
Nature cell biology 2006 Dec;8(12):1415-23
Nature cell biology 2006 Dec;8(12):1415-23
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- ITCH polyclonal antibody (Cat # PAB7215) (0.03 ug/mL) staining of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
